Structural and Biochemical Insights into Dimethylsulfoniopropionate Cleavage by Cofactor-Bound DddK from the Prolific Marine Bacterium Pelagibacter

摘要: Enormous amounts of the organic osmolyte dimethylsulfoniopropionate (DMSP) are produced in marine environments where bacterial DMSP lyases cleave it, yielding acrylate and climate-active gas dimethyl sulfide (DMS). SAR11 bacteria most abundant clade heterotrophic oceans play a key role catabolism. An important environmental factor affecting DMS generation via is availability metal ions because they essential cofactors for many these enzymes. Here we examine structure activity DddK presence various ions. We have established that containing double-stranded β-helical motif utilizes divalent as catalytic activity. However, nickel, an ion environments, adopts distorted octahedral coordination environment conferred highest lyase Crystal structures cofactor-bound reveal binding residues pro...