Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections
作者： R C Curran , J Gregory
关键词: Fluorescein isothiocyanate 、 Immunohistochemistry 、 Proteolytic enzymes 、 Chemistry 、 Antigen 、 Fixation (histology) 、 Pathology 、 Immunofluorescence 、 Fixative 、 Antibody 、 Molecular biology
摘要: A fluorescein isothiocyanate (FITC) technique and one based on peroxidase-antiperoxidase (PAP) were used to study the distribution of immunoglobulin (Ig) in cryostat paraffin sections human tonsil. Trypsin other proteolytic enzymes 'unmask' antigen sections. The effects processing, particularly fixation, immunohistochemical response tissues studied. FITC PAP methods detected Ig equally well. was same with both but method more informative. Formaldehyde-sucrose solution proved suitable for fixing immunohistochemistry than glutaraldehyde. revealed parraffin efficiently pepsin, papain, or pronase. Surface (s-Ig) could be demonstrated trypsinised less effectively Trypsinised were, however, intracellular (c-Ig) although performance improved by prior fixation a coagulative fixative.
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