Inhibitory activity of caffeoylquinic acids from the aerial parts of Artemisia princes on rat lens aldose reductase and on the formation of advanced glycation end products

摘要: Caffeoylquinic acids -3,4-di-O-caffeoylquinic acid (1); 1,3,5-tri-O-caffeoylquinic (2); and 3,4,5-tri-O-caffeoylqunic (3)- were isolated from an acetone-soluble fraction of the aerial parts Artemisia princes. Their structures determined spectroscopically using 1D- 2D-nuclear magnetic resonance (NMR) studies, as well by comparing NMR results with previously published structures. All isolates subjected to in vitro bioassays evaluate their efficacy inhibiting rat lens aldose reductase (RLAR) activity formation advanced glycation end products (AGEs). We found (2) be most potent AGE inhibitor, concentration that resulted 50% inhibition (IC50) was 22.18 ±1.46 mM, compared aminoguanidine chlorogenic controls, which had IC50 values 1,093.11±10.95 117.63±0.20 respectively. In RLAR assay, three caffeoylquinic have range 1.78-2.40 μM, demonstrating a 5- 10-fold greater quercetin control, value 17.91 μM.