Mapping of a vaccinia host range sequence by insertion into the viral thymidine kinase gene

摘要: A vaccinia virus mutant deleted of ca. 18 kilobase pairs at the left-hand end genome is unable to multiply on many human cell lines. To determine whether all or some sequences were responsible for host range property, corresponding region from wild-type DNA was cloned in three pieces into a transplacement vector containing thymidine kinase gene HindIII J fragment. The next step transfer these deletion by vivo homologous recombination around locus. Transfer one 5.2-kilobase-pair EcoRI fragment found restore phenotype mutant, thus demonstrating that only small portion 18-kilobase-pair contains function(s). This result also illustrates method initially devised inserting foreign genes useful studies genome.