Reaction rates of creatine kinase and ATP synthesis in the isolated rat heart. A 31P NMR magnetization transfer study.
作者： J A Bittl , J S Ingwall
DOI: 10.1016/S0021-9258(19)83652-9
关键词: Creatine 、 Magnetization transfer 、 ATP synthase 、 Chemical kinetics 、 Flux (metabolism) 、 Oxygen 、 Creatine kinase 、 Reaction rate 、 Biophysics 、 Chemistry 、 Stereochemistry
摘要: The NMR technique of magnetization transfer can be used to define intracellular reaction kinetics. In order determine the relationship between ATP synthesis and flux through creatine kinase in intact heart, we this measure isolated, isovolumic rat heart at five levels cardiac performance oxygen consumption. unidirectional rate constants (s-1) calculated from a two-site exchange model for both forward reverse reactions increased with As rate-pressure product varied 0 44.7 X 10(3) mm Hg/min consumption rose 5.9 45.8 mumol O2/g dry weight/min, kforward 0.27 1.30 kreverse 0.31 1.14. consumption, thus synthesis, took form Michaelis-Menten equation. Rates estimated were similar values longitudinal relaxation time phosphate (2.06 s), gamma-phosphorus atom (0.75 inorganic (0.81 s) did not change performance. These results show that myocardial energy via is closely coupled production.
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