Phosphorylation by Pho85 Cyclin-dependent Kinase Acts as a Signal for the Down-regulation of the Yeast Sphingoid Long-chain Base Kinase Lcb4 during the Stationary Phase
作者： Soichiro Iwaki , Akio Kihara , Takamitsu Sano , Yasuyuki Igarashi
关键词: Cyclin-dependent kinase complex 、 ASK1 、 Cell biology 、 Phosphorylation cascade 、 Mitogen-activated protein kinase kinase 、 Biochemistry 、 Biology 、 Cyclin-dependent kinase 、 Cyclin-dependent kinase 2 、 Cyclin-dependent kinase 1 、 MAP2K7
摘要: Sphingoid long-chain base 1-phosphates (LCBPs) act as bioactive lipid molecules in eukaryotic cells. In yeast, LCBPs are synthesized mainly by the kinase Lcb4p. Until now, regulatory mechanism for Lcb4p has been unclear. present study, we found that is post-translationally modified phosphorylation. Using a protein mutant yeast collection, further demonstrated cyclin-dependent Pho85p involved this functions number of cellular processes, especially response to environmental changes. Two 10 cyclins, Pcl1p and Pcl2p had overlapping phosphorylation Site-directed mutagenesis identified sites Ser(451) Ser(455). Additionally, pulse-chase experiments revealed degraded via ubiquitin-dependent pathway. The was stabilized Deltapho85 cells, suggesting acts signal degradation. down-regulated stationary phase cell growth, both ubiquitination appear be important process. Moreover, delivered vacuole degradation multivesicular body. Since forced accumulation results prolonged growth during phase, down-regulation may physiologically proper responses nutrient deprivation.
sci-hub.se 参考文章(55)
Jason Moffat, Dongqing Huang, Brenda Andrews, Functions of Pho85 cyclin-dependent kinases in budding yeast Progress in Cell Cycle Research. ,vol. 4, pp. 97- 106 ,(2000) , 10.1007/978-1-4615-4253-7_9
Pei-Jung Lu, Gerburg Wulf, Xiao Zhen Zhou, Peter Davies, Kun Ping Lu, The prolyl isomerase Pin1 restores the function of Alzheimer-associated phosphorylated tau protein. Nature. ,vol. 399, pp. 784- 788 ,(1999) , 10.1038/21650
K. C. Garcia, M. Degano, R. L. Stanfield, A. Brunmark, M. R. Jackson, P. A. Peterson, L. Teyton, I. A. Wilson, An alphabeta T cell receptor structure at 2.5 A and its orientation in the TCR-MHC complex. Science. ,vol. 274, pp. 209- 219 ,(1996) , 10.1126/SCIENCE.274.5285.209
Margaret M. LANTERMAN, Julie D. SABA, Characterization of sphingosine kinase (SK) activity in Saccharomyces cerevisiae and isolation of SK-deficient mutants. Biochemical Journal. ,vol. 332, pp. 525- 531 ,(1998) , 10.1042/BJ3320525
V Measday, L Moore, R Retnakaran, J Lee, M Donoviel, A M Neiman, B Andrews, A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase. Molecular and Cellular Biology. ,vol. 17, pp. 1212- 1223 ,(1997) , 10.1128/MCB.17.3.1212
Erin K. O’Shea, Marc E. Lenburg, Genetic Evidence for a Morphogenetic Function of the Saccharomyces cerevisiae Pho85 Cyclin-Dependent Kinase Genetics. ,vol. 157, pp. 39- 51 ,(2001) , 10.1093/GENETICS/157.1.39
V Measday, L Moore, J Ogas, M Tyers, B Andrews, The PCL2 (ORFD)-PHO85 cyclin-dependent kinase complex: a cell cycle regulator in yeast Science. ,vol. 266, pp. 1391- 1395 ,(1994) , 10.1126/SCIENCE.7973731
Kun Ping Lu, Steven D. Hanes, Tony Hunter, A human peptidyl-prolyl isomerase essential for regulation of mitosis Nature. ,vol. 380, pp. 544- 547 ,(1996) , 10.1038/380544A0
Nitai C Hait, Katsuhide Fujita, Robert L Lester, Robert C Dickson, Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes. FEBS Letters. ,vol. 532, pp. 97- 102 ,(2002) , 10.1016/S0014-5793(02)03636-0
Markus Babst, David J. Katzmann, Eden J. Estepa-Sabal, Timo Meerloo, Scott D. Emr, Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Developmental Cell. ,vol. 3, pp. 271- 282 ,(2002) , 10.1016/S1534-5807(02)00220-4