Analysis of parentage determination in Atlantic salmon (Salmo salar) using microsatellites

摘要: Microsatellite genetic markers are becoming increasing important tools in the investigation of alternate reproductive strategies wild plants and animals, implementation optimal breeding programs for endangered species, managed cultured populations. Overall, little attention is paid to frequency impact scoring errors mutations on resolution accuracy such analyses. Here, parentage 792 Atlantic salmon (Salmo salar) reared communally were determined using di- tetranucleotide microsatellites. Over 99·5% offspring could be unambiguously matched one set parents original 12 (1 × 1) experimental cross (each males uniquely crossed females) a simulated 36 (involving additional parents), over 80% 12 × 12 (all all females). Mutations rare (≈3·4 × 10−−4), though relatively common (2–3% per allele scored), with rate error varying among loci. Approximately 90% (or mutations) expected detected this analysis, those that not, fewer than 0·5% should lead false or incorrect determinations parentage. Based several indices, we expect greater 99·7% assayed their true parents.