Preservation of the protective group under alkaline conditions by using CaCl2. Applications in peptide synthesis
作者： Robert Pascal , Régine Sola
DOI: 10.1016/S0040-4039(98)00994-0
关键词: Combinatorial chemistry 、 Organic chemistry 、 Chemistry 、 Hydrolysis 、 Saponification 、 Alkaline hydrolysis 、 Group (periodic table) 、 Peptide synthesis 、 Peptide 、 Linker 、 Catalysis
摘要: Abstract CaCl 2 is shown to dramatically increase the lifetime of Fmoc protective group in alkaline iPrOH-H O but has little effect on hydrolytic processes. This enables efficient preparation Fmoc-protected peptide segments by saponification corresponding C-terminal methyl or benzyl esters. Similarly, protected prepared Fmoc/tBu solid-phase synthesis can be selectively released from support -catalyzed hydrolysis an N-acylurea-based linker.
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