Immobilization of naringinase in PVA-alginate matrix using an innovative technique.

摘要: A synthetic polymer, polyvinyl alcohol (PVA), a cheap and nontoxic polymer to organism, has been ascribed for biocatalyst immobilization. In this work PVA–alginate beads were developed with thermal, mechanical, chemical stability high temperatures (<80 °C). The combination of alginate bead treatment sodium sulfate not only prevented agglomeration but produced gel strength conferred enzyme protection from inactivation by boric acid. Naringinase Penicillium decumbens was immobilized in PVA (10%)–alginate three different sizes (1–3 mm), at concentrations (0.2–1.0%), these features investigated terms swelling ratio within the beads, activity, immobilization yield during hydrolysis naringin. pH temperature optimum 4.0 70 °C PVA–alginate-immobilized naringinase. highest naringinase activity (1%) 2 mm 80%, 70 °C. Michaelis constant (K Mapp) maximum reaction velocity (V maxapp) evaluated both free Mapp = 0.233 mM; V maxapp = 0.13 mM min−1) Mapp = 0.349 mM; maxapp = 0.08 mM min−1). residual followed eight consecutive batch runs retention 70%. After 6 weeks, upon storage acetate buffer 4 4 °C, retained 90% initial activity. These promising results are illustrative potential strategy system suggest that its application may be effectively performed entrapment other biocatalysts.