Comparative Proteomics Reveals that Phosphorylation of β Carbonic Anhydrase 1 Might be Important for Adaptation to Drought Stress in Brassica napus.

摘要: Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to stress were studied three cultivars. For cultivar 2AF009 with high tolerance, comparative proteomic analyses conducted determine molecular behind. Approximately 138 differentially abundant proteins (DAPs) 1232 phosphoproteins containing 4469 phosphopeptides identified. Furthermore, 337 547 phosphorylation sites demonstrated significant changes. These drought-responsive DAPs mainly involved signal transduction, photosynthesis, glutathione-ascorbate metabolism. Notably, 9 also identified as phosphoproteins, especially beta carbonic anhydrase 1 (βCA1), which was represented by eight distinct protein spots levels during stress. Tyr207 phosphorylated site βCA1 down-regulated at level stress, located substrate-binding active region three-dimensional (3D) structure. Moreover, inhibited CA activity. We concluded that most likely target affecting enzyme activity, might be important for response rapeseed. The study provided a new clue B.napus.