Insulin and other growth factors induce binding of the ternary complex and a novel protein complex to the c-fos serum response element.
作者： P J Blackshear , M W Roe , M J Thompson , R K Malik
DOI: 10.1016/S0021-9258(17)31939-7
关键词: Cell biology 、 Phorbol 、 Biology 、 Multiprotein complex 、 Serum Response Element 、 Ternary complex 、 Biochemistry 、 Serum response factor 、 Nuclear protein 、 Growth factor 、 Electrophoretic mobility shift assay
摘要: Rapid, transient induction of c-fos transcription follows treatment cells with insulin and other growth factors. This is mediated through the serum response element (SRE), which binds factor (SRF), as well accessory factors such p62 ternary complex factor. Using a gel shift assay we found that formation increased transiently within 2 min or phorbol ester several insulin-sensitive cell lines. However, mutations prevented this did not inhibit insulin- ester-stimulated in these We also identified novel SRF-containing multiprotein forms on SRE following insulin, ester, treatment. Formation complex, called band 3, occurred rapidly transiently, time course parallel to transcription. Band 3 formed gamma-actin zif268/3 probes. Methylation carboxy ethylation interference analysis, extensive mutagenesis, suggest only SRF directly contacts forming 3. appears involve protein-protein interactions between nuclear protein(s) may play role stimulation
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