Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes

摘要: We report on the fabrication and characterisation of a gold-nanoparticle (AuNP)-based mediatorless sugar/oxygen biofuel cell (BFC) operating in neutral sugar-containing buffers human physiological fluids, such as blood plasma. First, Corynascus thermophilus cellobiose dehydrogenase (CtCDH) Myrotheciumverrucaria bilirubin oxidase (MvBOx), used anodic cathodic bioelements, respectively, were immobilised gold electrodes modified with 20 nm AuNPs. Detailed optimisation new CDH/AuNP-based bioanode performed following fundamental parameters obtained: (i) redox potential haem-containing centre enzyme was measured to be 75 mV vs. NHE, (ii) surface coverage CtCDH found 0.65 pmol cm(-2) corresponding sub-monolayer thiol-modified AuNPs by enzyme, (iii) turnover number for calculated ca. 0.5 s(-1), (iv) maximal current densities high 40 mu A registered buffers. Second, both biomodified electrodes, namely CtCDH/AuNP-based MvBOx/AuNP-based biocathode, combined into functional BFC designed biodevices carefully investigated. The characteristics mediator-, separator- membrane-less, miniature phosphate buffer; an open-circuit voltage 0.68 V, maximum power density 15 W at 0.52 V blood; V. 3 0.45 respectively. estimated half-lives >12, <8, <2h buffer, plasma, blood, basic BFCs significantly improved compared previously biodevices, because usage three-dimensional AuNP-modified electrodes. (C) 2011 Elsevier B.V. All rights reserved. (Less)