Gene Enrichment Analysis Reveals Major Regulators of Mycobacterium tuberculosis Gene Expression in Two Models of Antibiotic Tolerance.

摘要: The development of antibiotic tolerance is believed to be a major factor in the lengthy duration current tuberculosis therapies. In study, we have modeled vitro by exposing Mycobacterium two distinct stress conditions: progressive hypoxia and nutrient starvation [phosphate-buffered saline (PBS)]. We then studied bacterial transcriptional response using RNA-seq employed bioinformatics approach identify important regulators, which was facilitated novel Regulon Enrichment Test (RET). A total 17 transcription (TF) regulons were enriched gene set 16 starvation, with 12 both conditions. Using same analyze previously published expression datasets, found that three M. (Rv0023, SigH, Crp) commonly induced conditions also among our data. These regulators are worthy further study determine their potential role maintenance following exposure.