Cdna Production from Cells After Laser Microdissection
作者： Birgit Liss
DOI:
关键词: Laser capture microdissection 、 Complementary DNA 、 Rapid amplification of cDNA ends 、 DNA 、 Buffer (optical fiber) 、 Molecular biology 、 RNA extraction 、 Biology 、 Lysis 、 Microdissection
摘要: The invention relates to a novel method for producing cDNA from individual cells after microdissection. inventive is inexpensive, comprises few steps and can be carried out also by less skilled laboratory personnel. obviates the time-consuming risky step of RNA isolation allowing lysis synthesis in same reaction vessel buffer solution which yields reliable contamination-free results. composed NP40, carrier DNA Super RNAsin©, as well dNTPs primers.
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