Synthesis of the vasoactive intestinal peptide (VIP) I. The C-terminal cyanogen bromide fragment
作者： Miklos Bodanszky , Yakir S. Klausner , Viktor Mutt
DOI: 10.1016/0045-2068(73)90004-7
关键词: Stereochemistry 、 Vasoactive intestinal peptide 、 Fragment (computer graphics) 、 Chemistry 、 Enzymatic hydrolysis 、 Cyanogen bromide
摘要: Abstract The C-terminal cyanogen bromide fragment of VIP, Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH 2 (all l ), was synthesized to provide evidence for the correctness sequence proposed by Mutt and Said ( 1 ). synthesis this hendecapeptide (VIP 18–28 ) carried out coupling Z-Ala-Val-(Z)Lys (Z)Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH . After removal protecting groups purification, synthetic material indistinguishable from natural on paper chromatograms electropherograms. Their identity further confirmed comparison products formed enzymatic hydrolysis.
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