Binary transformation systems based on 'shooter' mutants of Agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.
作者： V. Mihálka , E. Balázs , I. Nagy
DOI: 10.1007/S00299-003-0597-6
关键词: Genetically modified crops 、 Genetics 、 Mutant 、 Transfer DNA 、 Apical dominance 、 Transformation (genetics) 、 Gene 、 Biology 、 Agrobacterium tumefaciens 、 Marker gene
摘要: A simple transformation procedure with a positive selection scheme using the expression of isopentenyl transferase (ipt) gene transfer DNA (T-DNA) 'shooter' mutants Agrobacterium tumefaciens was elaborated. After comparing several we found that particular strains frequently produced phenotypically normal shoots after co-culturing tobacco leaf explants. Shoots selected for phenotype showed apical dominance and could be rooted same efficiency as non-transformed shoots. When binary vectors were introduced into these strains, stably integrated vector T-DNA sequences in some regenerants, which under non-selective conditions on growth-regulator-free medium. Such transformants typically lacked ipt gene. Normal looking also tomato, muskmelon sweet pepper.
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R. A. Jefferson, T. A. Kavanagh, M. W. Bevan, GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal. ,vol. 6, pp. 3901- 3907 ,(1987) , 10.1002/J.1460-2075.1987.TB02730.X
P M Klapwijk, T Scheulderman, R A Schilperoort, Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. Journal of Bacteriology. ,vol. 136, pp. 775- 785 ,(1978) , 10.1128/JB.136.2.775-785.1978
D J Garfinkel, E W Nester, Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. Journal of Bacteriology. ,vol. 144, pp. 732- 743 ,(1980) , 10.1128/JB.144.2.732-743.1980
Ana Cristina Miranda Brasileiro, Jean-Charles Lepl�, Joris Muzzin, Dalila Ounnoughi, Marie-France Michel, Lise Jouanin, An alternative approach for gene transfer in trees using wild-type Agrobacterium strains. Plant Molecular Biology. ,vol. 17, pp. 441- 452 ,(1991) , 10.1007/BF00040638
G. F. Barry, S. G. Rogers, R. T. Fraley, L. Brand, Identification of a cloned cytokinin biosynthetic gene Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 4776- 4780 ,(1984) , 10.1073/PNAS.81.15.4776
S. Endo, T. Kasahara, K. Sugita, E. Matsunaga, H. Ebinuma, The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco (Nicotiana tabacum cv. Petite Havana SRI) Plant Cell Reports. ,vol. 20, pp. 60- 66 ,(2001) , 10.1007/S002990000279
G. Ditta, S. Stanfield, D. Corbin, D. R. Helinski, Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 7347- 7351 ,(1980) , 10.1073/PNAS.77.12.7347
Géraldine Bonnard, Bruno Tinland, François Paulus, Ernö Szegedi, Léon Otten, Nucleotide sequence, evolutionary origin and biological role of a rearranged cytokinin gene isolated from a wide host range biotype III Agrobacterium strain. Molecular Genetics and Genomics. ,vol. 216, pp. 428- 438 ,(1989) , 10.1007/BF00334387
H. Ebinuma, K. Sugita, E. Matsunaga, M. Yamakado, Selection of marker-free transgenic plants using the isopentenyl transferase gene Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 2117- 2121 ,(1997) , 10.1073/PNAS.94.6.2117
Attila Szász, Giuseppe Nervo, Miklós Fári, Screening for in vitro shoot-forming capacity of seedling explants in bell pepper (Capsicum annuum L.) genotypes and efficient plant regeneration using thidiazuron. Plant Cell Reports. ,vol. 14, pp. 666- 669 ,(1995) , 10.1007/BF00232735