Recommended safe practices for using the neurotoxin MPTP in animal experiments.

摘要: Abstract The metabolism and distribution of the parkinsonian syndrome inducing neurotoxin MPTP has been studied in non-human primates mice housed controlled environmental chambers. 14C6-MPTP was prepared injected at concentrations normally employed for lesioning experiments (30 mg/kg mice, 0.3 monkeys). All interior surfaces chambers which could be reached by animals or their excreta were contaminated with radiolabeled metabolites. Vapor born unmetabolized negligible, although significant amounts found (less than equal to 15% dose) small from rhesus monkeys 2%). Procedures minimize contact animal fur, bedding should protect investigators working over extended periods. Permanganate oxidation effectively detoxifies solutions MPTP. MPTP, MPP+, common synthetic intermediates, products MPTP's are not mutagenic as measured a Salmonella-microsome assay.