作者: H. Melero , N. Garcia-Giralt , J. Fernández , A. Díez-Pérez , J.M. Guilemany
DOI: 10.3233/BME-140989
关键词: Ceramic 、 Coating 、 Viability assay 、 Materials science 、 High velocity 、 Polystyrene 、 In vitro 、 Oxy-fuel 、 Thermal spraying 、 Chemical engineering
摘要: Hydroxyapatite coatings obtained by plasma-spraying have been used for many years to improve biological perfor- mance of bone implants, but several studies drawn attention the problems arising from high temperatures and lack mechanical properties. In this study, is substituted velocity oxy-fuel (HVOF) spray, with lower reached, TiO2 added in low amounts hydroxyapatite order Four conditions tested evaluate which are those better Viability proliferation tests, as well differentiation assays morphology observation, performed human osteoblast cultures onto studied coatings. The hydroxyapatite-TiO2 maintain good cell viability proliferation, especially cases higher amorphous phase amount specific surface, promote excellent differentiation, a ALP these than polystyrene controls. Observation SEM corroborates behaviour. conclusion, alternative industrially, an interesting issue would be improving behaviour worst cases, turn show