Interaction of Grb2 via its Src homology 3 domains with synaptic proteins including synapsin I

摘要: Abstract Grb2 is a 25-kDa adaptor protein composed of Src homology 2 (SH2) domain and two flanking 3 (SH3) domains. One function Grb2 to couple tyrosine-phosphorylated proteins (through its SH2 domain) downstream effectors SH3 domains). Using an overlay assay, we have identified four major Grb2-binding in synaptic fractions. These interact with wild-type but not containing point mutations each domains corresponding the loss mutants Caenorhabditis elegans homologue sem-5. Two proteins, mSos dynamin, were previously shown bind Grb2. The third 145 kDa brain specific our knowledge has been described. fourth synapsin I. Dynamin required for vesicle endocytosis I thought mediate interaction vesicles presynaptic cytomatrix. data suggest that Grb2, or other domains, may play role regulation exo/endocytotic cycle therefore neurotransmitter release.