The 2.0 Å crystal structure of a heterotrimeric G protein
作者： David G Lambright , John Sondek , Andrew Bohm , Nikolai P Skiba , Heidi E Hamm
DOI: 10.1038/379311A0
关键词: G12/G13 alpha subunits 、 Heterotrimeric G protein 、 G protein 、 G beta-gamma complex 、 Biophysics 、 GTP-Binding Protein beta Subunits 、 Biochemistry 、 Protein subunit 、 GTP-Binding Protein gamma Subunits 、 Biology 、 GTP-Binding Protein alpha Subunits
摘要: The structure of a heterotrimeric G protein reveals the mechanism nucleotide-dependent engagement α and βγ subunits that regulates their interaction with receptor effector molecules. involves two distinct interfaces dramatically alters conformation αbut not theβγ subunits. location known sites for posttranslational modification coupling suggest plausible orientation respect to membrane surface an activated heptahelical receptor.
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E. Leberer, D. Dignard, L. Hougan, D.Y. Thomas, M. Whiteway, Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling. The EMBO Journal. ,vol. 11, pp. 4805- 4813 ,(1992) , 10.1002/J.1460-2075.1992.TB05586.X
Lubert Stryer, James B. Hurley, Bernard K.-K. Fung, Transducin and the cyclic GMP phosphodiesterase of retinal rod outer segments Methods in Enzymology. ,vol. 96, pp. 617- 627 ,(1983) , 10.1016/S0076-6879(83)96054-8
Bruce R. Conklin, Zvi Farfel, Kevin D. Lustig, David Julius, Henry R. Bourne, Substitution of three amino acids switches receptor specificity of Gqα to that of Giα Nature. ,vol. 363, pp. 274- 276 ,(1993) , 10.1038/363274A0
T.A. Neubert, R.S. Johnson, J.B. Hurley, K.A. Walsh, The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated. Journal of Biological Chemistry. ,vol. 267, pp. 18274- 18277 ,(1992) , 10.1016/S0021-9258(19)36955-8
T Higashijima, K M Ferguson, P C Sternweis, M D Smigel, A G Gilman, Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins. Journal of Biological Chemistry. ,vol. 262, pp. 762- 766 ,(1987) , 10.1016/S0021-9258(19)75851-7
M E Linder, I H Pang, R J Duronio, J I Gordon, P C Sternweis, A G Gilman, Lipid modifications of G protein subunits.: Myristoylation of Goα increases its affinity for βγ Journal of Biological Chemistry. ,vol. 266, pp. 4654- 4659 ,(1991) , 10.1016/S0021-9258(20)64372-1
W A Maltese, J D Robishaw, Isoprenylation of C-terminal cysteine in a G-protein gamma subunit. Journal of Biological Chemistry. ,vol. 265, pp. 18071- 18074 ,(1990) , 10.1016/S0021-9258(17)44715-6
Irene Garcia-Higuera, Thomas C. Thomas, Fei Yi, Eva J. Neer, Intersubunit surfaces in G protein alpha beta gamma heterotrimers. Analysis by cross-linking and mutagenesis of beta gamma. Journal of Biological Chemistry. ,vol. 271, pp. 528- 535 ,(1996) , 10.1074/JBC.271.1.528
B K Fung, C R Nash, Characterization of transducin from bovine retinal rod outer segments. II. Evidence for distinct binding sites and conformational changes revealed by limited proteolysis with trypsin. Journal of Biological Chemistry. ,vol. 258, pp. 10503- 10510 ,(1983) , 10.1016/S0021-9258(17)44484-X
E Lee, R Taussig, A G Gilman, The G226A mutant of Gs alpha highlights the requirement for dissociation of G protein subunits. Journal of Biological Chemistry. ,vol. 267, pp. 1212- 1218 ,(1992) , 10.1016/S0021-9258(18)48416-5