Cloning and characterization of 29 tetranucleotide and two dinucleotide polymorphic microsatellite loci from the endangered marbled murrelet (Brachyramphus marmoratus)
作者： MARY BETH REW , M. ZACHARIAH PEERY , STEVEN R. BEISSINGER , MARTINE BERUBE , JEFFREY D. LOZIER
DOI: 10.1111/J.1471-8286.2006.01206.X
关键词: Brachyramphus 、 Population 、 Evolutionary biology 、 Biology 、 Critically endangered 、 Microsatellite 、 Loss of heterozygosity 、 Endangered species 、 Locus (genetics) 、 Seabird 、 Ecology
摘要: We developed 31 novel, polymorphic microsatellite loci in the marbled murrelet (Brachyramphus marmoratus), a critically endangered seabird. Variability was tested on 15 individuals from Santa Cruz, California population, with each locus characterized by two to 12 alleles. Observed levels of heterozygosity ranged 0.13 0.93. These provide valuable means assessing population structure and demographic parameters this species, which may be critical its conservation across fragmented habitat.
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sci-hub.se 参考文章(6)
Kary B. Mullis, Fred A. Faloona, Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology. ,vol. 155, pp. 335- 350 ,(1987) , 10.1016/0076-6879(87)55023-6
Travis C. Glenn, Nancy A. Schable, Isolating microsatellite DNA loci. Methods in Enzymology. ,vol. 395, pp. 202- 222 ,(2005) , 10.1016/S0076-6879(05)95013-1
D. PAETKAU, W. CALVERT, I. STIRLING, C. STROBECK, Microsatellite analysis of population structure in Canadian polar bears Molecular Ecology. ,vol. 4, pp. 347- 354 ,(1995) , 10.1111/J.1365-294X.1995.TB00227.X
William R. Rice, ANALYZING TABLES OF STATISTICAL TESTS Evolution. ,vol. 43, pp. 223- 225 ,(1989) , 10.1111/J.1558-5646.1989.TB04220.X
Markus Schuelke, An economic method for the fluorescent labeling of PCR fragments Nature Biotechnology. ,vol. 18, pp. 233- 234 ,(2000) , 10.1038/72708
M. Raymond, F. Rousset, GENEPOP (Version 1.2): Population Genetics Software for Exact Tests and Ecumenicism Journal of Heredity. ,vol. 86, pp. 248- 249 ,(1995) , 10.1093/OXFORDJOURNALS.JHERED.A111573