Existence of NO-triggered vagal afferent activation in the rat gastric mucosa
作者： Hisayuki Uneyama , Akira Niijima , Akihiko Kitamura , Kunio Torii
DOI: 10.1016/J.LFS.2009.10.009
关键词: Nitric oxide synthase 、 Gastric mucosa 、 Internal medicine 、 Stomach 、 Chemistry 、 Sodium nitroprusside 、 Regulation of gastric function 、 Nitric oxide 、 Gastroenterology 、 Enterochromaffin cell 、 Endocrinology 、 Vagus nerve 、 General Pharmacology, Toxicology and Pharmaceutics 、 General Biochemistry, Genetics and Molecular Biology 、 General Medicine
摘要: Abstract Aim We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by gastric vagus nerve, and we proposed that stomach, like duodenum, could “taste” nutrients. Nitric synthase (NOS) is widely distributed mucosa, but physiological role NO not well understood. In this study, investigated functional coupling vagal nerve endings mucosa. Main methods For electrophysiological recordings, male Sprague-Dawley rats were anesthetized with urethane, afferent responses rat branches to a donor monitored. Key findings Intravenous application 100 µg/kg sodium nitroprusside (SNP) transiently increased discharges ventral vagus, which was followed rapid changes blood pressure. High doses SNP (> 300 µg/kg, i.v.) showed biphasic increase discharges. Secondary activation continued even after pressure returned basal levels. SNP-evoked abolished depletion using p -cholorophenylalanine inhibited pre- post-treatment 3 antagonist granisetron. Significance These pharmacological results strongly indicate NO-triggered coupled
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sci-hub.se 参考文章(35)
R M Palmer, S Moncada, E A Higgs, NITRIC OXIDE: PHYSIOLOGY, PATHOPHYSIOLOGY, AND PHARMACOLOGY Pharmacological Reviews. ,vol. 43, pp. 109- 142 ,(1991)
K J LePard, R L Stephens, Serotonin inhibits gastric acid secretion through a 5-hydroxytryptamine1-like receptor in the rat. Journal of Pharmacology and Experimental Therapeutics. ,vol. 270, pp. 1139- 1144 ,(1994)
H Ahlman, L DeMagistris, M Zinner, B. Jaffe, Release of immunoreactive serotonin into the lumen of the feline gut in response to vagal nerve stimulation. Science. ,vol. 213, pp. 1254- 1255 ,(1981) , 10.1126/SCIENCE.6168020
Håkan Ahlman, Annica Dahlström, Vagal mechanisms controlling serotonin release from the gastrointestinal tract and pyloric motor function Journal of The Autonomic Nervous System. ,vol. 9, pp. 119- 140 ,(1983) , 10.1016/0165-1838(83)90136-4
Lavern J. Weber, p-chlorophenylalanine depletion of gastrointestinal 5-hydroxytryptamine☆ Biochemical Pharmacology. ,vol. 19, pp. 2169- 2172 ,(1970) , 10.1016/0006-2952(70)90317-5
Hisayuki Uneyama, Akira Niijima, Tatsuro Tanaka, Kunio Torii, Receptor subtype specific activation of the rat gastric vagal afferent fibers to serotonin. Life Sciences. ,vol. 72, pp. 415- 423 ,(2002) , 10.1016/S0024-3205(02)02271-3
Jörg Glatzle, Catia Sternini, Carla Robin, Tilman T. Zittel, Helen Wong, Joseph R. Reeve, Helen E. Raybould, Expression of 5-HT3 receptors in the rat gastrointestinal tract Gastroenterology. ,vol. 123, pp. 217- 226 ,(2002) , 10.1053/GAST.2002.34245
Tatsuya Tamura, Isamu Sano, Minoru Satoh, Akiyoshi Mizumoto, Zen Itoh, Pharmacological characterization of 5-hydroxytryptamine-induced motor activity (in vitro) in the guinea pig gastric antrum and corpus European Journal of Pharmacology. ,vol. 308, pp. 315- 324 ,(1996) , 10.1016/0014-2999(96)00312-3
K. Racké, A. Reimann, H. Schwörer, H. Kilbinger, Regulation of 5-HT release from enterochromaffin cells. Behavioural Brain Research. ,vol. 73, pp. 83- 87 ,(1995) , 10.1016/0166-4328(96)00075-7
Sara Calatayud, Dolores Barrachina, Juan V. Esplugues, Nitric oxide: relation to integrity, injury, and healing of the gastric mucosa. Microscopy Research and Technique. ,vol. 53, pp. 325- 335 ,(2001) , 10.1002/JEMT.1100