Decreased production of interferon-gamma by human neonatal cells. Intrinsic and regulatory deficiencies.

摘要: Human neonatal lymphocytes produced little macrophage activation factor in response to mitogens. This correlated with decreased production of interferon-gamma (IFN gamma): adult lymphokines contained 894.2 +/- 177.1 U/ml, whereas cord and peripheral 66.9 17.0 116.7 29.6 U/ml by bioassay. Results radioimmunoassay (RIA) for IFN gamma were similar. In contrast, the interleukin 2 content was greater (P less than 0.01) that blood similar adults. Interleukin 1 receptor expression affinity cells. Interleukins amounts comparable those did not increase or Neonatal cells contain intracellular degrade exogenous IFN. Excess suppressor activity found cultures. Addition alpha, 10,000-50,000 phorbol myristate acetate (PMA) mononuclear monocytes PMA T increased compared stimulated concanavalin A (ConA) alone. Nevertheless, under optimal conditions (T + Con A), much more (1,360 261 RIA) (122 37 U/ml). Staphylococcal enterotoxin (SEA) cell at low densities; nevertheless, SEA 1,341 350 U/ml) (350 33 Decreased appears be due both differences their intrinsic capacity produce regulatory mechanisms.