Norepinephrine transporter immunoblotting and radioligand binding in cocaine abusers.

摘要: The norepinephrine transporter (NET) is a membrane protein responsible for transporting extracellular norepinephrine. cocaine and tricyclic antidepressant-sensitive NET belongs to family of sodium chloride coupled transporters that include the monoamines dopamine serotonin amino acids GABA glycine. regional distribution has been defined by synaptosomal uptake autoradiographic approaches in rodent primate brain. However, not well characterized human brain due overall low abundance expressed axon terminals. Recently, immunolocalization studies have used identify cytoplasmic epitope We report here on characteristics drug interactions with native postmortem Antisera raised against 17-amino acid peptide from N-terminus hNET recognized an 80 kDa species cerebral cortex. Chronic exposure upregulated expression [3H]nisoxetine binding sites insular cortex brains addicts. These results demonstrate immunologic radioligand afford specific labeling transport