TH‐C‐220‐12: Biochemical Radiation Response of Irradiated Human Tumour Cells Observed via Raman Spectroscopy

摘要: Purpose: To use non‐invasive single‐cell Raman spectroscopy (RS) and principal component analysis (PCA) to analyze radiation‐induced biochemical changes in human tumour cell lines, correlate results with known radiosensitivity. Methods: Six derived from prostate (DU145, PC3, LNCaP), breast (MDA‐MB‐231 MCF7) lung (H460), were irradiated single fractions (15, 30 or 50 Gy) of 6 MV photons. Remaining live cells harvested for RS at 0, 24, 48, 72 hours post‐irradiation, along unirradiated controls. Single‐cell spectra acquired 20 per sample a microscope utilizing 785 nm excitation laser. All (200 line) post‐processed, the total data set each line was analyzed PCA using standard algorithms. Results: One unique detected by identification statistically significant score distributions samples, as compared first 24 post‐irradiation. Correlation between components different lines shows that these radiation response signatures fall into 3 distinct categories: R1 (H460 MCF7), R2 PC3), R3 (DU145 LNCaP). These categories segregate according radiosensitivity p53 gene status. The are radioresistant (SF2 > 0.6), whereas radiosensitive < 0.5). further status, verified cycle Conclusions: This study demonstrates is powerful tool radiobiological investigations, promising technique advancing field personalized therapy.