Evaluation of in vitro activities of extracellular enzymes from Aspergillus species isolated from corneal ulcer/keratitis.
作者： Annanthode Balakrishnan Sangeetha , Ahmed Abdel-hadi , Anamangadan Shafeeq Hassan , Coimbatore Subramanian Shobana , Sreeram Suresh
DOI: 10.1016/J.SJBS.2019.11.023
关键词: Eye infection 、 Lipase 、 Extracellular 、 Keratinase 、 Protease 、 Elastase 、 Fungal keratitis 、 Aspergillus 、 Biology 、 Microbiology
摘要: Mycotic/fungal keratitis is a suppurative, generally ulcerative infection of the cornea. The filamentous fungi, Aspergillus spp. are second leading cause mycotic keratitis, particularly in India. produce range extracellular enzymes that used to break down complex molecules and for growth reproduction, also survival on/in host organism. current study was designed with an objective screen vitro enzyme activity Fusarium isolates from patients correlate same as putative virulence factor. Extracellular viz., deoxyribonuclease (DNase), protease, lipase, elastase, keratinase, etc., produced by have key role keratomycosis hence their (n=85) activities were investigated. It found that, majority protease (n = 75; 88 % 85) followed lipase =59; 69% 85), DNase 35; 41 elastase (n=26; 31 keratinase 13; 15 85). indices (EAI) DNase, ranged between 1.01 1.98, whereas EAI varied 1.26 1.92. showed maximum 1.98 lowest value 1.01, respectively. may potential onset progression
psau.edu.sa参考文章(40)
H G Dimova, G S Schultz, G B van Setten, K C Balch, J P Barletta, M T Moser, G A Stern, G Angella, Inhibition of pseudomonal ulceration in rabbit corneas by a synthetic matrix metalloproteinase inhibitor. Investigative Ophthalmology & Visual Science. ,vol. 37, pp. 20- 28 ,(1996)
Arumugam Mythili, Yendranbam Randhir Babu Singh, Ravindran Priya, Anamangadan Shafeeq Hassan, Palanisamy Manikandan, Kanesan Panneerselvam, Venkatapathy Narendran, Coimbatore Subramanian Shobana, None, In vitro and comparative study on the extracellular enzyme activity of molds isolated from keratomycosis and soil. International Journal of Ophthalmology. ,vol. 7, pp. 778- 784 ,(2014) , 10.3980/J.ISSN.2222-3959.2014.05.07
Ivana Mravii, Iva Dekaris, Nikica Gabri, Ivana Romac, Vlade Glavota, Emilija Mlinari-, An Overview of Fungal Keratitis and Case Report on Trichophyton Keratitis Keratitis. pp. 1- 15 ,(2012) , 10.5772/33695
Palanisamy Manikandan, János Varga, Sándor Kocsubé, Raghavan Anita, Rajaraman Revathi, Tibor Mihály Németh, Venkatapathy Narendran, Csaba Vágvölgyi, Kanesan Panneer Selvam, Coimbatore Subramanian Shobana, Yendremban Randhir Babu Singh, László Kredics, Epidemiology of Aspergillus keratitis at a tertiary care eye hospital in South India and antifungal susceptibilities of the causative agents. Mycoses. ,vol. 56, pp. 26- 33 ,(2013) , 10.1111/J.1439-0507.2012.02194.X
M Borg, R Rüchel, Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infection and Immunity. ,vol. 56, pp. 626- 631 ,(1988) , 10.1128/IAI.56.3.626-631.1988
P E Kolattukudy, J D Lee, L M Rogers, P Zimmerman, S Ceselski, B Fox, B Stein, E A Copelan, Evidence for possible involvement of an elastolytic serine protease in aspergillosis. Infection and Immunity. ,vol. 61, pp. 2357- 2368 ,(1993) , 10.1128/IAI.61.6.2357-2368.1993
M. A. DUDLEY, E. W. CHICK, CORNEAL LESIONS PRODUCED IN RABBITS BY AN EXTRACT OF FUSARIUM MONILIFORME. Archives of Ophthalmology. ,vol. 72, pp. 346- 350 ,(1964) , 10.1001/ARCHOPHT.1964.00970020346012
J. Friedrich, H. Gradišar, D. Mandin, J. P. Chaumont, Screening fungi for synthesis of keratinolytic enzymes Letters in Applied Microbiology. ,vol. 28, pp. 127- 130 ,(1999) , 10.1046/J.1365-2672.1999.00485.X
F.C. Odds, Candida albicans proteinase as a virulence factor in the pathogenesis of Candida infections Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology. ,vol. 260, pp. 539- 542 ,(1985) , 10.1016/S0176-6724(85)80069-9
W.-S. Zhu, K. Wojdyla, K. Donlon, P.A. Thomas, H.I. Eberle, Extracellular proteases of Aspergillus flavus: Fungal keratitis, proteases, and pathogenesis Diagnostic Microbiology and Infectious Disease. ,vol. 13, pp. 491- 497 ,(1990) , 10.1016/0732-8893(90)90081-6