作者: Dapeng Li , Robert J Edwards , Kartik Manne , David R Martinez , Alexandra Schäfer
DOI: 10.1101/2020.12.31.424729
关键词: In vitro 、 Cytokine 、 Virology 、 Virus 、 Medicine 、 Antibody 、 Disease 、 Fc receptor 、 Bronchoalveolar lavage 、 In vivo
摘要: SARS-CoV-2 neutralizing antibodies (NAbs) protect against COVID-19, making them a focus of vaccine design. A safety concern regarding is whether they mediate disease enhancement. Here, we isolated potent NAbs the receptor-binding domain (RBD) and N-terminal (NTD) spike protein from individuals with acute or convalescent history SARS-CoV-1 infection. Cryo-electron microscopy RBD NTD demonstrated function-specific modes antibody binding. Select also Fc receptor-γ (FcγR)-mediated enhancement virus infection in vitro , while five non-neutralizing mediated FcγR-independent However, both infection-enhancing protected challenge non-human primates mice. One 30 monkeys infused enhancing had lung pathology bronchoalveolar lavage cytokine evidence suggestive enhanced disease. Thus, these assessments antibody-mediated do not necessarily indicate biologically relevant vivo