Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
作者： Keisuke Ito , Sohei Ito , Tatsuro Shimamura , Yasuaki Kawarasaki , Keiko Abe
DOI: 10.1107/S1744309110029714
关键词: Glucansucrase 、 Crystallization 、 Pathogen 、 Strain (chemistry) 、 Streptococcus mutans 、 Chemistry 、 Glycoside hydrolase 、 Escherichia coli 、 Microbiology 、 X ray analysis
摘要: Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members glycoside hydrolase family 70 (GH 70). Data crystal structure GH have yet to be reported. Here, glucansucrase GTF-SI from S. was overexpressed Escherichia coli strain BL21 (DE3), purified homogeneity and crystallized using hanging-drop vapour-diffusion method. Orthorhombic crystals belonging space group P21212 were obtained. A diffraction data set collected 2.1 A resolution.
sci-hub.se 参考文章(11)
Zbyszek Otwinowski, Wladek Minor, Processing of X-ray diffraction data collected in oscillation mode Methods in Enzymology. ,vol. 276, pp. 307- 326 ,(1997) , 10.1016/S0076-6879(97)76066-X
Y Yamashita, W H Bowen, H K Kuramitsu, Molecular analysis of a Streptococcus mutans strain exhibiting polymorphism in the tandem gtfB and gtfC genes. Infection and Immunity. ,vol. 60, pp. 1618- 1624 ,(1992) , 10.1128/IAI.60.4.1618-1624.1992
E. Newbrun, C.I. Hoover, Gwen J. Walker, Inhibition by acarbose, nojirimycin and 1-deoxynojirimycin of glucosyltransferase produced by oral streptococci. Archives of Oral Biology. ,vol. 28, pp. 531- 536 ,(1983) , 10.1016/0003-9969(83)90186-3
M. Tamesada, S. Kawabata, T. Fujiwara, S. Hamada, Synergistic effects of streptococcal glucosyltransferases on adhesive biofilm formation. Journal of Dental Research. ,vol. 83, pp. 874- 879 ,(2004) , 10.1177/154405910408301110
E.Ann MacGregor, Hans M. Jespersen, Birte Svensson, A circularly permuted α-amylase-type α/β-barrel structure in glucan-synthesizing glucosyltransferases FEBS Letters. ,vol. 378, pp. 263- 266 ,(1996) , 10.1016/0014-5793(95)01428-4
Akio Yanagida, Tomomasa Kanda, Masayuki Tanabe, Fumio Matsudaira, José Geraldo Oliveira Cordeiro, Inhibitory Effects of Apple Polyphenols and Related Compounds on Cariogenic Factors of Mutans Streptococci Journal of Agricultural and Food Chemistry. ,vol. 48, pp. 5666- 5671 ,(2000) , 10.1021/JF000363I
Vincent Monchois, René-Marc Willemot, Pierre Monsan, Glucansucrases: mechanism of action and structure–function relationships Fems Microbiology Reviews. ,vol. 23, pp. 131- 151 ,(1999) , 10.1111/J.1574-6976.1999.TB00394.X
Sacha A. F. T. van Hijum, Slavko Kralj, Lukasz K. Ozimek, Lubbert Dijkhuizen, Ineke G. H. van Geel-Schutten, Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria Microbiology and Molecular Biology Reviews. ,vol. 70, pp. 157- 176 ,(2006) , 10.1128/MMBR.70.1.157-176.2006
Pei-Feng Liu, Wen-Hong Zhu, Chun-Ming Huang, Vaccines and Photodynamic Therapies for Oral Microbial-Related Diseases Current Drug Metabolism. ,vol. 10, pp. 90- 94 ,(2009) , 10.2174/138920009787048365
Hyun Koo, Pedro L. Rosalen, Jaime A. Cury, Yong K. Park, William H. Bowen, Effects of Compounds Found in Propolis on Streptococcus mutans Growth and on Glucosyltransferase Activity Antimicrobial Agents and Chemotherapy. ,vol. 46, pp. 1302- 1309 ,(2002) , 10.1128/AAC.46.5.1302-1309.2002