An Allosteric Path to Transcription Termination
作者： Vitaly Epshtein , Christopher J. Cardinale , Andrei E. Ruckenstein , Sergei Borukhov , Evgeny Nudler
DOI: 10.1016/J.MOLCEL.2007.10.011
关键词: RNA polymerase 、 Transcription (biology) 、 Intrinsic termination 、 Terminator (genetics) 、 Genetics 、 Biophysics 、 Biology 、 Stem-loop 、 RNA polymerase II 、 Termination factor 、 Antitermination
摘要: Transcription termination signals in bacteria occur RNA as a strong hairpin followed by stretch of U residues at the 3' terminus. To release transcript, polymerase (RNAP) is thought to translocate forward without synthesis. Here we provide genetic and biochemical evidence supporting an alternative model which extensive conformational changes across enzyme lead translocation. In this model, flexible parts exit channel (zipper, flap, zinc finger) assist initial step folding (nucleation). The then invades RNAP main channel, causing RNA:DNA hybrid melting, structural catalytic site, DNA-clamp opening induced interaction with G(trigger)-loop. Our results envision elongation complex structure, not rigid body, establish basic principles pathway that are likely be universal prokaryotic eukaryotic systems.
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