Utilization of Seafood Processing By-Products for Production of Proteases by Paenibacillus sp. TKU052 and Their Application in Biopeptides' Preparation.

摘要: Microbial fermentation of by-products is a renewable and efficient technique in the development range useful products. In this study, protease synthesis by Paenibacillus sp. TKU052 was carried out on culture media containing some common seafood processing (SPBPs) as sole source carbon nitrogen (C/N). The most suitable C/N nutrition for production proteases found to be 3.0% (w/v) demineralized crab shells powder (deCSP) maximal enzyme activity 4.41 ± 0.16 U/mL detected third day culture. Two (P1 P2) with similar molecular weight 31 kDa were successfully isolated purified from 3-day deCSP-containing medium. Both P1 P2 exhibited highest gelatin hydrolysis at pH 6 60 °C. hydrolysates catalyzed evaluated biological activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, angiotensin-I converting (ACE) inhibition, prebiotic activities. expressed 31.76–43.95% DPPH scavenging 31.58–36.84% ACE inhibitory activity, which higher than those gelatin. Gelatin also showed growth-enhancing effect Bifidobacterium bifidum BCRC 14615 an increase 135.70–147.81%. short, could potential strain utilize shell wastes produce bio-active peptides’ preparation.