Corticosterone actions on the hippocampal brain-derived neurotrophic factor expression are mediated by exon IV promoter.

摘要: Brain-derived neurotrophic factor (BDNF) expression is strongly regulated by adrenocorticosteroids via activated gluco- and mineralocorticoid receptors. Four separate promoters are located upstream of the BDNF noncoding exons I to IV may thus be involved in adrenocorticosteroid-mediated gene regulation. In adrenalectomised rats, corticosterone (10 mg/kg s.c.) induces a robust down-regulation both mRNA protein levels hippocampus peaking at 2-8 h. To study role individual response, we employed exon-specific riboprobe situ hybridisation as well real-time polymerase chain reaction (PCR) dentate gyrus. We found down-regulation, mainly exon protein-coding V, nearby all hippocampal subregions, but II was only down-regulated Exon III transcripts were not affected treatment. The results could confirmed with PCR It appears if promoter major target for corticosterone-mediated transcriptional regulation hippocampus.