Chapter 7 HPLC of Diacylglycerol and Phospholipase C Sensitive Glycerolipids in Microsomes of Normal Tissues and Dystrophic Muscle (Type Duchenne)
作者： Bernd Rüstow , Harald Rabe , Dietrich Kunze
DOI: 10.1016/S0301-4770(08)60601-6
关键词: Chromatography 、 De novo synthesis 、 Derivatization 、 Enzyme 、 Biochemistry 、 Diacylglycerol kinase 、 Chemistry 、 Glycerophospholipids 、 Biosynthesis 、 Microsome 、 Phospholipase C
摘要: Publisher Summary This chapter discusses the method of high pressure liquid chromatography (HPLC) separation individual diacylglycerols (DG) species. As DG moieties glycerophospholipids can be released by phospholipase C, this technique opens up entire field to a sensitive assessment species patterns these lipid classes too. The derivatization with alpha-naphthylisocyanate yielding DG-urethanes allows and quantification 19 peaks representing 23 HPLC. sensitivity is in range 3 pmol for an Using labeled precursors, measurement newly synthesized C glycerolipids. A comparison endogenous pattern PC from different organs shows differences between two glycerolipids organs. PG obtained microsomes mitochondria lung tissue lead conclusion that both organelles participate biosynthesis surfactant lipids. study de novo synthesis demonstrates progress advantage analysis relation use exogenously added investigation acyl selectivity enzymes involved some aspects organization regulation pathway.
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