Genome-wide identification of the entire 90 glutathione S-transferase (GST) subfamily genes in four rotifer Brachionus species and transcriptional modulation in response to endocrine disrupting chemicals.

摘要: Genome-wide identification of glutathione S-transferase (GST), a major phase II detoxification enzyme, was investigated in four different aquatic model rotifer species Brachionus koreanus, B. plicatilis, rotundiformis, and calyciflorus. GSTs are ubiquitous antioxidant enzymes that play versatile function including cellular detoxification, stress alleviation, production the radical conjugates. Among rotifers, rotundiformis found with least number GST genes (total 19 genes), whereas other three shared 23 to 24 genes. identified genes, belonging cytosolic superfamily, expansion sigma classes mainly occurs through tandem duplication, resulting tandem-arrayed gene clusters on chromosomes. Overall, discovered this study highest class, zeta, alpha, omega descending order. With integration phylogenetic analysis xenobiotic-mediated mRNA expression patterns along previous enzymatic activities, functional divergence among species-specific clearly observed. This covers full marine one fresh-water their important role environmental ecotoxicology.