Nitric Oxide and Hydrogen Peroxide Mediate Wounding-Induced Freezing Tolerance through Modifications in Photosystem and Antioxidant System in Wheat.

摘要: Mechanical wounding is a common stress caused by herbivores or manual and natural manipulations, whereas its roles in acclimation response to wide spectrum of abiotic stresses remain unclear. The present work showed that local mechanical enhanced freezing tolerance untreated systemic leaves wheat plants (Triticum aestivum L.), meanwhile the signal molecules hydrogen peroxide (H2O2) nitric oxide (NO) were accumulated systemically. Pharmacological study wounding-induced NO synthesis was substantially arrested pretreatment with scavengers reactive oxygen species an inhibitor NADPH oxidase (Respiratory burst homolog, RBOH). On contrary, H2O2 accumulation not sensitive synthetic inhibitors scavenger, indicating acts upstream transduction pathways. Cytochemical vascular tissues localizations approved RBOH-dependent as long-distance response. Transcriptome analysis revealed 279 genes up-regulated treated freezing, but alone. Importantly, freezing- significantly enriched categories “photosynthesis” “signaling”. These results strongly supported primary can induce through H2O2, further modifications photosystem antioxidant system.