Automated analysis of free l-carnitine and total short-chain acylcarnitine

摘要: Disclosed is a method of assaying free L-carnitine and total short-chain acylcarnitine in blood. The uses essentially protein-free samples plasma or serum comprises (a) obtaining sample ultrafiltrated serum, (b) converting all carnitine to acetylcarnitine using an enzymatic reaction, (c) coenzyme A liberated the reaction thiophenylate, (d) evaluating spectrophotometrically determine amount thiophenylate present, (e) fresh hydrolyzing convert carnitine, (f) repeating steps through (d). All following ultrafiltration are carried out within pre-programmed, automated centrifugal analyzer. use this measure only also disclosed, as kit for carrying analysis acylcarnitine.