Two-photon imaging
作者： C. Ricard , J.A. Coles , R. Serduc , B. van der Sanden , P. Verant
DOI: 10.1016/B978-008045046-9.00863-9
关键词: Femtosecond 、 Confocal 、 Neuroimaging 、 Second-harmonic generation 、 Optical microscope 、 Microscopy 、 Microscope 、 Optics 、 Two-photon excitation microscopy 、 Chemistry
摘要: A two-photon microscope is basically a scanning confocal that uses for excitation near-infrared light delivered in intense, femtosecond pulses. It allows imaging with the spatial resolution of conventional optical (<1 μm) but can perform such through several hundred micrometers brain tissue. In this article, physical principles are explained, and cortex living mouse neurons, glia, microvasculature described. Finally, advantages microscopy studying neuropathologies including tumors summarized.
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