Antiparasitic Efficacy of Curcumin Against Besnoitia besnoiti Tachyzoites in vitro.

摘要: Besnoitia besnoiti is the causative agent of bovine besnoitiosis. B. infections lead to reduced fertility and productivity in cattle causing high economic losses, not only Europe, but also Asia Africa. Mild severe clinical signs, such as anasarca, oedema, orchitis, hyperkeratosis, characteristic skin mucosal cysts, are due tachyzoite bradyzoite replication intermediate host tissues. So far, there no commercially available effective drugs against this parasite. Curcumin, a polyphenolic compound from Curcuma longa rhizome well-known for its antioxidant, anti-inflammatory, immunomodulatory anti-protozoan effects. Hence, objective study was evaluate effects curcumin on viability, motility, invasive capacity, proliferation tachyzoites replicating primary umbilical vein endothelial cells (BUVEC) vitro. Functional inhibition assays revealed that treatments reduce viability induce lethal up 57% (IC50 5.93 μM). Referring general significant dose-dependent were observed. Interestingly, dampened helical gliding twirling activities whilst longitudinal motility significantly affected. In addition, pretreatments resulted reduction cell invasion detected by rates at 1 day p. i. These findings demonstrate feeding with rhizomes may represent new strategy besnoitiosis treatment.