Paraquat induces apoptosis in human lymphocytes: Protective and rescue effects of glucose, cannabinoids and insulin-like growth factor-1
作者： Marlene Jimenez Del Rio , Carlos Velez-Pardo
DOI: 10.1080/08977190801984205
关键词: Cytoprotection 、 Acridine orange 、 Oxidative stress 、 Ethidium bromide 、 Paraquat 、 Programmed cell death 、 Apoptosis 、 Formazan 、 Pharmacology 、 Biology 、 Biochemistry 、 Clinical biochemistry 、 Cell biology 、 Endocrinology
摘要: In order to establish causal or protective treatments for Parkinson's disease (PD), it is necessary identify the cascade of deleterious events that lead dysfunction and death dopaminergic neurons. Paraquat (PQ) a pesticide used as xenobiotic compound model PD. However, mechanism(s) PQ-induced cell cytoprotection in single are still unknown. this study, lymphocytes were treated with (0.1–1 mM) PQ. Apoptotic morphology was assessed acridine orange/ethidium bromide staining. Further evaluation included (i) superoxide radicals, reflected by nitroblue tetrazolium reduction formazan, (ii) production hydrogen peroxide, rhodamine-positive fluorescent cells, (iii) generation hydroxyl dimethylsulfoxide melatonin √OH scavengers, (iv) activation and/or translocation NF-κB, p53 c-Jun transcription factors showed immunocytochemical staining, ammonium pyrrolidinedithiocar...
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