Localization and molecular signaling pathways of prostaglandin receptor subtypes in the eye

摘要: The eicosanoids are derived from membrane fatty acids. Prostaglandins (PGs), produced the metabolism of arachidonic acid, members eicosanoid family. exert a broad range biological effects by interacting with plasma membrane-bound receptors that coupled to guanine nucleotide-binding proteins (G proteins). topical application prostaglandins results in long-lasting reduction intraocular pressure (lOP) mammalian eyes, including humans. distribution and fimction prostaglandin receptor subtypes within eye not clearly understood. studies presented this dissertation focused at addressing three specific aims which have been designed test following hypothesis; involved maintenance through modulation aqueous humor production regulation outflow pathways. Subtype selective antibodies individual prostanoid generated enable study these tissue, cellular molecular level. Utilization series pharmacological techniques identified areas lOP. functional responses obtained primary cultures human trabecular meshwork bovine ciliary epithelium provide evidence for involvement both outflow.