Probing the folding transition state of ubiquitin mutants by temperature-jump-induced downhill unfolding.
作者： Hoi Sung Chung , Ali Shandiz , Tobin R. Sosnick , Andrei Tokmakoff
DOI: 10.1021/BI801603E
关键词: Equilibrium unfolding 、 Chemical physics 、 Temperature jump 、 Protein folding 、 Folding (chemistry) 、 Phi value analysis 、 Crystallography 、 Microsecond 、 Nanosecond 、 Chemistry 、 Phase (matter)
摘要: Crucial to revealing mechanistic details of protein folding is a characterization the transition state ensemble and its structural dynamics. To probe ubiquitin thermal unfolding, we examine unfolding dynamics kinetics wild-type mutant using time-resolved nonlinear infrared spectroscopy after nanosecond temperature jump. We observe spectral changes on two different time scales. A fast nonexponential microsecond phase attributed downhill from region, which induced by shift barrier due rapid change. Slow millisecond arise thermally activated kinetics. Mutants that stabilize or destabilize β strands III−V lead decreased increased amplitude phase, indicating disruption weakening these occurs in state. Unfolding features microseconds milliseconds can be explained temperature-dependent c...
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sci-hub.se 参考文章(48)
George I. Makhatadze, Peter L. Privalov, Energetics of protein structure. Advances in Protein Chemistry. ,vol. 47, pp. 307- 425 ,(1995) , 10.1016/S0065-3233(08)60548-3
Bryan A. Krantz, Tobin R. Sosnick, Engineered metal binding sites map the heterogeneous folding landscape of a coiled coil. Nature Structural & Molecular Biology. ,vol. 8, pp. 1042- 1047 ,(2001) , 10.1038/NSB723
Jonathan P.L. Cox, Philip A. Evans, Leonard C. Packman, Dudley H. Williams, Derek N. Woolfson, Dissecting the Structure of a Partially Folded Protein Journal of Molecular Biology. ,vol. 234, pp. 483- 492 ,(1993) , 10.1006/JMBI.1993.1600
Samuel Krimm, Jagdeesh Bandekar, Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Advances in Protein Chemistry. ,vol. 38, pp. 181- 364 ,(1986) , 10.1016/S0065-3233(08)60528-8
George S. Hammond, A Correlation of Reaction Rates Journal of the American Chemical Society. ,vol. 77, pp. 334- 338 ,(1955) , 10.1021/JA01607A027
Bryan A. Krantz, Tobin R. Sosnick, Distinguishing between two-state and three-state models for ubiquitin folding. Biochemistry. ,vol. 39, pp. 11696- 11701 ,(2000) , 10.1021/BI000792+
Wei Yuan Yang, Martin Gruebele, Folding at the speed limit Nature. ,vol. 423, pp. 193- 197 ,(2003) , 10.1038/NATURE01609
Mourad Sadqi, David Fushman, Victor Muñoz, Atom-by-atom analysis of global downhill protein folding. Nature. ,vol. 442, pp. 317- 321 ,(2006) , 10.1038/NATURE04859
Christopher M. Cheatum, Andrei Tokmakoff, Jasper Knoester, Signatures of beta-sheet secondary structures in linear and two-dimensional infrared spectroscopy. Journal of Chemical Physics. ,vol. 120, pp. 8201- 8215 ,(2004) , 10.1063/1.1689637
Hoi Sung Chung, Munira Khalil, Adam W. Smith, Andrei Tokmakoff, Transient two-dimensional IR spectrometer for probing nanosecond temperature-jump kinetics. Review of Scientific Instruments. ,vol. 78, pp. 063101- ,(2007) , 10.1063/1.2743168