作者: Roberta Galuppi , Benedetto Morandi , Silvia Agostini , Sara Dalla Torre , Monica Caffara
关键词: DNA extraction 、 Malassezia 、 External Ear Canals 、 Malassezia cuniculi 、 Population 、 Veterinary medicine 、 Mycobiota 、 Biology 、 Isolation (microbiology) 、 Agar
摘要: Malassezia spp. have rarely been reported in rodents and lagomorphs. In 2011, cuniculi was described two rabbits. Further microscopic studies showed M. cuniculi-like yeasts more than 50% of samples from rabbits’ ear canals, but no isolation made. The present study details the presence tries to typify it canals healthy Seventy-eight half-breed rabbits rural farms 98 companion dwarf northern Italy were considered. A first attempt screen swabs performed by cultural examination on Sabouraud Glucose Agar (SGA), modified Dixon (mDA) Leeming Notman (LNA). Additionally, eight further microscopically positive for cells, used both LNA medium nine its variants DNA extraction, PCR sequencing. observation screened 168 highlighted one or external (58.3%). Rabbits meat production frequently diagnosed pet (P = 0.001), young ones often compared older 3 months 0.027). No yeast growth observed culture. From selected rabbits, failed mediums. Sequences ~300 bp fragments 18s rDNA, obtained swabs, 99.9% identity with phylotype 131 human canals. As Malassezia-like half examined population, colonization meatus can be considered as physiological results outline how much remains discovered a component skin mycobiota that use culture alone is not best choice detect