Raddeanin A promotes apoptosis and ameliorates 5-fluorouracil resistance in cholangiocarcinoma cells

作者: Shuang-Shuang Guo , Ying Wang , Qing-Xia Fan

DOI: 10.3748/WJG.V25.I26.3380

关键词: ApoptosisMedicineViability assayCellCancer researchWee1MetastasisCell cycleCyclin D1Bile duct cancerGastroenterologyGeneral Medicine

摘要: Background Bile duct cancer is characterized by fast metastasis and invasion has been regarded as one of the most aggressive tumors due to absence effective diagnosis at an early stage. Therefore, it in urgent demand explore novel diagnostic approaches therapeutic strategies for bile improve patient survival. Raddeanin A (RA) extracted from anemone raddeana regel demonstrated play antitumor roles various cancers. Aim To investigate effects RA treatment on cells. Methods In this study, four cholangiocarcinoma cell lines (RBE, LIPF155C, LIPF178C, LICCF) treated with were used test viability. The RA-associated functional analysis, 5-fluorouracil (5-Fu) effectiveness well cycle- apoptosis-related protein expression investigated. Results reduced viability a dose-dependent pattern lines, migration colony formation abilities also impaired RBE LIPF155C lines. sensitized 5-Fu enhanced cholangiocarcinoma. Also, decreased Wee1, while combinational effect expressions cyclooxygenase-2, B lymphoma 2, Wee1 but increased levels Bax, cyclin D1, E. Conclusion Taken together, results suggest that acts anti-cancer agent enhancer cells via regulating multiple cycle proteins. This finding provides clues exploring drug cancer.

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