Binding of polybenzamides to DNA: studies by DNase I and chlorambucil interference footprinting and comparison with Hoechst 33258

摘要: The DNA sequence-specific binding ability of polybenzamide minor groove ligands was investigated. These were compared with the known binder Hoechst 33258, using both DNase I footprinting and chlorambucil interference footprinting. monocationic derivative showed some sequence specific to A/T-rich sequences, as shown by footprinting, but results for biscationic inconclusive. A general non-specific inhibition cleavage at high drug concentrations observed, suggesting these compounds had a low affinity 33258. Using complementary technique, displayed clear preference sites containing least three consecutive adenines and, in contrast analogue, lesser mixed A/T sequences.