Prevalence and genetic variability of Anaplasma phagocytophilum in wild rodents from the Italian alps

摘要: Human granulocytic anaplasmosis is a zoonotic bacterial disease with increasing relevance for public health in Europe. The understanding of its sylvatic cycle and identification competent reservoir hosts are essential improving risk models planning preventative measures. In 2012 we collected single ear biopsy punches from 964 live-trapped rodents the Province Trento, Italy. Genetic screening Anaplasma phagocytophilum (AP) was carried out by PCR amplification fragment 16S rRNA gene. Fifty-two (5.4%) samples tested positive: 49/245 (20%) bank vole (Myodes glareolus) 3/685 (0.4%) yellow-necked mouse (Apodemus flavicollis). From these 52 positive samples, generated 38 groEL 39 msp4 sequences. Phylogenetic analysis confirmed existence distinct rodent strain AP. Our results confirm circulation specific AP our study area; moreover, they provide further evidence marginal role A. flavicollis compared to M. glareolus as host this pathogen.