Pigment Diversity in Freshwater Phytoplankton. I. A Comparison of Spectrophotometric and Paper Chromatographic Methods

摘要: Spectrophotometric and paper chromatographic analyses of the pigments in phytoplankton were made from early spring till end summer two small Dutch freshwater lakes. It was found that pigment diversity cannot be adequately estimated by MARGALEF'S ratio nor polychromatic spectrophotometric methods. The detected with method were: chlorophyll-a, chlorophyll-b, chlorophyll-c, phaeophytin-a (traces), phaeophorbide-a, Mg-containing chlorophyll-derivatives, carotene, lutein, violaxanthin, neoxanthin fucoxanthin, diadinoxanthin, diatoxanthin peridinin keto-carotenoids (traces). is suggested to distinguish between a richness-component an evenness-component diversity.