Hydrogen Peroxide Head Preparation: Enabling Cuttings and Anatomic Studies of Skull Base Dura Mater and Arachnoid Without Use of Drilling.

摘要: Introduction Anatomic pieces that are preserved using formaldehyde allow us to undertake high-quality skull base studies. However, extensive drilling is often necessary, which can lead damages the dura mater and thus arachnoid. Formaldehyde hydrogen peroxide soften bone, which, in turn, be easily cut with a scalpel or removed curette. After having discovered this technique by chance, our aim was establish study protocol of without use drill. Methods Ten heads were set 10% formalin solution then injected colored latex. Five subsequently bleached 20% (HPS). The following studied weekly: 1) macroscopic modification mater, arachnoid brain; 2) histology; 3) computed tomography scans; 4) calcium concentration screenings weekly. Results several weeks (mean 6.1, range 5–8 weeks), all HPS specimens flexible, similar rubber consistence. Geometrical bone cuts could made while preserving surrounding anatomic structure (cranial nerves, vascular elements). Histologically, dural preserved. cadavers appear radiologically demineralized. We note significant augmentation after 1 month, 6 weeks, 2 months compared day 0. Conclusions softening probably caused decalcification from corrosive chemicals present solution, ease cutting geometrically, useful for workshop