Protection effect of nicotinamide on cardiomyoblast hypoxia/re-oxygenation injury: study of cellular mitochondrial metabolism.
作者： He Wang , Xiaoping Liang , Guoan Luo , Mingyu Ding , Qionglin Liang
DOI: 10.1039/C6MB00108D
关键词: Nicotinamide adenine dinucleotide 、 Nicotinamide 、 NAD+ kinase 、 Succinate dehydrogenase 、 Heart metabolism 、 Reactive oxygen species 、 Biology 、 Mitochondrion 、 Pharmacology 、 Biochemistry 、 Metabolism
摘要: Hypoxia/re-oxygenation (H/R) injury is an important cause of heart failure and results in a critical metabolism dysfunction. In this paper, the cytoprotective effect nicotinamide adenine dinucleotide (NAD) precursor was evaluated using vitro model cardiac H/R injury. Nicotinamide (0-20 mM) applied to myoblast cell line H9c2 which subjected hypoxia (12, 24, 36 h) followed by re-oxygenation process (0, 4, 8, 12 h). Cell viability measured, mitochondrial metabolites were extracted then measured HPLC/MS/MS. The present study showed that could down-regulate NADH/NAD ratio maintain NAD-dependent processes. Furthermore, aberrant decrease fumarate levels increase succinate observed group, demonstrated be caused nicotinamide-induced dehydrogenase (SDH) inhibition. These suggest exerts protective on cardiomyoblasts against H/R-induced through both regulation reduction reactive oxygen species generation via SDH
rsc.org
sci-hub.se 参考文章(32)
Fumiko Sadanaga-Akiyoshi, Hiroshi Yao, Sei-ichi Tanuma, Tatsuo Nakahara, Jong Soon Hong, Setsuro Ibayashi, Hideyuki Uchimura, Masatoshi Fujishima, Nicotinamide attenuates focal ischemic brain injury in rats: With special reference to changes in nicotinamide and NAD+ levels in ischemic core and penumbra Neurochemical Research. ,vol. 28, pp. 1227- 1234 ,(2003) , 10.1023/A:1024236614015
E J Griffiths, A P Halestrap, Mitochondrial non-specific pores remain closed during cardiac ischaemia, but open upon reperfusion. Biochemical Journal. ,vol. 307, pp. 93- 98 ,(1995) , 10.1042/BJ3070093
Myeong Jin Yoon, Mitsukuni Yoshida, Sean Johnson, Akiko Takikawa, Isao Usui, Kazuyuki Tobe, Takashi Nakagawa, Jun Yoshino, Shin-ichiro Imai, SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice Cell Metabolism. ,vol. 21, pp. 706- 717 ,(2015) , 10.1016/J.CMET.2015.04.002
Arianna Cattelan, Giulio Ceolotto, Sergio Bova, Mattia Albiero, Maniselvan Kuppusamy, Sara De Martin, Andrea Semplicini, Gian Paolo Fadini, Saula Vigili de Kreutzenberg, Angelo Avogaro, NAD(+)-dependent SIRT1 deactivation has a key role on ischemia-reperfusion-induced apoptosis. Vascular Pharmacology. ,vol. 70, pp. 35- 44 ,(2015) , 10.1016/J.VPH.2015.02.004
Yong Wang, Chun Li, Wenjing Chuo, Zhongyang Liu, Yulin Ouyang, Dong Li, Jing Han, Yan Wu, Shuzhen Guo, Wei Wang, None, Integrated proteomic and metabolomic analysis reveals the NADH-mediated TCA cycle and energy metabolism disorders based on a new model of chronic progressive heart failure. Molecular BioSystems. ,vol. 9, pp. 3135- 3145 ,(2013) , 10.1039/C3MB70263D
Katalin Módis, Domokos Gerő, Rita Stangl, Olivér Rosero, Attila Szijártó, Gábor Lotz, Petra Mohácsik, Petra Szoleczky, Ciro Coletta, Csaba Szabó, None, Adenosine and inosine exert cytoprotective effects in an in vitro model of liver ischemia-reperfusion injury. International Journal of Molecular Medicine. ,vol. 31, pp. 437- 446 ,(2013) , 10.3892/IJMM.2012.1203
Martin KLINGENBERG, Hagai ROTTENBERG, Relation between the gradient of the ATP/ADP ratio and the membrane potential across the mitochondrial membrane. FEBS Journal. ,vol. 73, pp. 125- 130 ,(1977) , 10.1111/J.1432-1033.1977.TB11298.X
David Siegel, Hjalmar Permentier, Rainer Bischoff, Controlling detrimental effects of metal cations in the quantification of energy metabolites via ultrahigh pressure-liquid chromatography-electrospray-tandem mass spectrometry by employing acetylacetone as a volatile eluent modifier. Journal of Chromatography A. ,vol. 1294, pp. 87- 97 ,(2013) , 10.1016/J.CHROMA.2013.04.029
Bing Luo, Karsten Groenke, Ralf Takors, Christian Wandrey, Marco Oldiges, Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry Journal of Chromatography A. ,vol. 1147, pp. 153- 164 ,(2007) , 10.1016/J.CHROMA.2007.02.034
Da-Li Tong, Dong-Xia Zhang, Fei Xiang, Miao Teng, Xu-Pin Jiang, Jing-Ming Hou, Qiong Zhang, Yue-Sheng Huang, Nicotinamide pretreatment protects cardiomyocytes against hypoxia-induced cell death by improving mitochondrial stress. Pharmacology. ,vol. 90, pp. 11- 18 ,(2012) , 10.1159/000338628