Live-Cell Migration and Adhesion Turnover Assays

摘要: Fluorescence microscopy has revolutionized the way live-cell imaging is achieved. At same time, it also potentially harmful to a living specimen. Therefore, specimen must be monitored for viability and health before, during, after sessions. Methods monitoring cell will discussed in this chapter. Another key successful minimize light exposure as much possible. A summary of strategies minimizing including maximizing throughput microscope sensitivity detection presented. Various fluorescence techniques are presented with focus on how delivered sample (i.e., density) pros cons use specimens. The reader directed other publications that go into these topics more detail. described prepare samples single migration assays, measure rates (e.g., bright-field, semi-automated, automated), focal adhesion turnover rates. Details correct images background intensity field-illumination uniformity artifacts quantitative described. Overall, chapter helpful scientists who interested live specimens using techniques. It particular interest anyone wanting perform imaging, rates, dynamics.