Live cell interferometry reveals cellular dynamism during force propagation.
作者： Jason Reed , Joshua J. Troke , Joanna Schmit , Sen Han , Michael A. Teitell
DOI: 10.1021/NN700303F
关键词: Flow cytometry 、 Interferometry 、 Cell fixation 、 Live cell imaging 、 Biology 、 Microscopy 、 Cell 、 Atomic force microscopy 、 Biophysics 、 Cytoskeleton 、 Cell biology
摘要: Cancer and many other diseases are characterized by changes in cell morphology, motion, mechanical rigidity. However, live cytology, stimulus-induced morphologic typically take 10−30 min to detect. Here, we employ live-cell interferometry (LCI) visualize the rapid response of a whole stimulation, on time scale seconds, detect cytoskeletal remodeling behavior within 200 s. This involved small, content miniscule shape; it would be difficult with conventional or phase contrast microscopy alone is beyond dynamic capability AFM. We demonstrate that LCI provides rapid, quantitative reconstruction body no labeling. an advantage over traditional flow cytometry, which require surface tagging and/or destructive fixation for labeling
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