A bacterial endosymbiont enables fungal immune evasion during fatal mucormycete infection

作者: Herbert Itabangi , Poppy C. S. Sephton-Clark , Xin Zhou , Georgina P. Starling , Zamzam Mahamoud

DOI: 10.1101/584607

关键词: Bacterial genome sizeRhizopus microsporusPhagocytosisPhagosome maturationSpore germinationImmune systemMacrophageMucoralesBiologyMicrobiology

摘要: Abstract Environmentally ubiquitous fungal spores of the Mucorales order cause acute invasive infections through germination and evasion mammalian host immune system. Early phagocyte control spore plays a key role in controlling infection, yet swelling evade phagocytosis an unknown mechanism. Here we investigate clinical isolate Rhizopus microsporus reveal bacterial endosymbiont, Ralsonia pickettii, pathogenesis. Analysis rates wild type cured isolates demonstrates for endosymbiont cell disruption cytoskeleton phagosome maturation. Further analysis secreted products revealed presence previously uncharacterized secondary metabolite whose production is induced by fungus. genome condition-dependent RNAseq implicate cryptic I polyketide synthase. Subsequent zebrafish larvae model infection demonstrate suppressing macrophage neutrophil recruitment to site infection. Finally, that this has implications clearance immunocompetent murine Together, these findings identify first time pathogenesis during animal

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